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    Article Outlines Chondroitin Testing Protocol

    ...

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    11.06.14
    An article outlining testing protocols for chondroitin purity has been published in the peer-reviewed Journal of AOAC INTERNATIONAL.
     
    “Electrophoretic Separation of Impurities in Chondroitin Sulfate and Identification of Certain Adulterants that Interfere with the Cetylpyridinium Chloride Titration Assay” was authored by James Neal-Kababick, Director of Flora Research Laboratories, Jana Hildreth, director of Technology and Scientific Affairs of Synutra Pure, Synutra’s president Weiguo Zhang, Dr. Gabriel Giancaspro, vice president of Foods, Dietary Supplements and Herbal Medicines at the United States Pharmacopeia (USP), Dr. Kristie Adams, who is a nuclear magnetic resonance (NMR) spectroscopist at USP, Dr. Joseph Betz, director, Analytical Methods and Reference Materials Program, Office of Dietary Supplements, National Institutes of Health, and the late Dr. Mark Roman, director of Tampa Bay Analytical Research Laboratories, to whom the research paper is dedicated. 
     
    The most commonly used chondroitin assay method, cetylpyridinium chloride (CPC) titration, can be fooled by various known adulterants. This article illustrates why CPC should be used only after cellulose acetate membrane electrophoresis (CAME) first determines purity. CAME is a complementary methodology to CPC that has been in the USP monograph for chondroitin sulfate for years. An inexpensive, simple, and effective procedure, CAME can effectively deter the practice of adulterating chondroitin ingredients with known adulterants.
     
    For more information on the article, visit:  http://ingentaconnect.com/content/aoac/jaoac/pre-prints/content-9706Zhang
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